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I propose a research plan that includes two longitudinal cohorts, one HIV-infected and one HIV-negative, and a randomized trial to assess the effectiveness of a behavioral intervention to prevent substance abuse in HIV-positive youth. The principal investigator completed an MPH degree in Epidemiology and a Master's in Social Work at the Johns Hopkins School of Public Health, and is currently completing a Doctoral degree in Public Health at the Johns Hopkins Bloomberg School of Public Health. The Johns Hopkins Institutional Review Board (IRB) has granted the PI permission to conduct the randomized trial described in this proposal. This research plan will allow the PI to build on this previous work, and take her research skills to the next level, as outlined in the following career development and research aims. In Aim 1, we will examine morbidity and mortality in HIV-infected and -negative children in a prospective cohort study. 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